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Affinity-Based Methods in Drug-Target Discovery

[ Vol. 16 , Issue. 1 ]

Author(s):

Gabriela Rylova, Tomas Ozdian, Lakshman Varanasi, Miroslav Soural, Jan Hlavac, Dusan Holub, Petr Dzubak and Marian Hajduch   Pages 60 - 76 ( 17 )

Abstract:


Target discovery using the molecular approach, as opposed to the more traditional systems approach requires the study of the cellular or biological process underlying a condition or disease. The approaches that are employed by the “bench” scientist may be genetic, genomic or proteomic and each has its rightful place in the drug-target discovery process. Affinity-based proteomic techniques currently used in drug-discovery draw upon several disciplines, synthetic chemistry, cell-biology, biochemistry and mass spectrometry. An important component of such techniques is the probe that is specifically designed to pick out a protein or set of proteins from amongst the varied thousands in a cell lysate. A second component, that is just as important, is liquid-chromatography tandem massspectrometry (LC-MS/MS). LC-MS/MS and the supporting theoretical framework has come of age and is the tool of choice for protein identification and quantification. These proteomic tools are critical to maintaining the drug-candidate supply, in the larger context of drug discovery.

Keywords:

Affinity purification, drug-target discovery, immobilization, mass-spectrometry, probe, quantification.

Affiliation:

Institute of Molecular and Translational Medicine Faculty of Medicine and Dentistry Palacky University in Olomouc, Hnevotinska 5, 779 00 Olomouc, Czech Republic.



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